SOS Allergo · I understand & manage
GRAT: testing the glove actually used
Glove Repeated Application Test — instructions for use

Why is this test used?
You have hand eczema and regularly wear gloves. A GRAT complements patch tests by checking whether the glove you actually use can reproduce a skin reaction. It does not replace the assessment of immediate latex allergy.
In this sheet
To complete on the PDF sheet
- Patient
- Glove / brand / reference
- Start date
- How the glove is used
Instructions for use
1 — Cut the glove
Cut a piece measuring at least 3 × 3 cm from the suspected glove. For a disposable glove, prepare a new piece for each night if possible.
2 — Mark out an area of healthy skin
On the inner forearm, choose skin without eczema or a wound and draw a square matching the size of the glove piece.
3 — Apply the glove piece overnight
At bedtime, place the inside surface of the glove against the skin and secure it with the tape or device indicated by the team. Leave it in place for about 6 to 8 hours, then remove it in the morning.
4 — Repeat on the same area
Unless your doctor gives different instructions: apply once every night for 10 consecutive nights, always to the same area. For a reusable glove, follow the instructions provided by the team.
Monitoring over 10 nights
Each morning, tick the box if no reaction has appeared. If a reaction occurs, immediately follow the red warning box below.
These boxes stay only in your browser and are not saved.
Stop the test and contact the team if a reaction appears
Marked redness, severe itching, spots or blisters, eczema resembling the eczema on your hands, or a reaction spreading beyond the test area. Take a photograph if possible. Never deliberately intensify the test.
How is the GRAT interpreted?
A positive GRAT supports the glove’s responsibility but does not identify the responsible chemical on its own. Patch tests, including an appropriate rubber series, remain useful. A negative GRAT makes the glove less likely to be responsible but does not always rule it out completely.
Latex or chemicals in the glove?
The GRAT mainly investigates delayed contact dermatitis caused by the glove. It does not replace assessment for immediate latex allergy. Many cases of glove eczema are caused by rubber accelerators, including in some latex-free nitrile gloves.
After the test
If the glove is involved, the aim is to find protection that suits your work and is compatible with your allergy, rather than to remove all protection.
Instructions from the team
- Duration each night in hours
- Maximum number of nights
- Next appointment
- Doctor / team
- Contact
SOS Allergo resources
Main references
- Lamouroux C, et al. Contact Dermatitis. 2024;90:528-530.
- Clément A, et al. Contact Dermatitis. 2023;89:143-152.
- Hansen A, et al. Allergol Select. 2021;5:335-344.
- Johansen JD, et al. Contact Dermatitis. 1998;39:95-96.